PrimePCR™ Probe Assay: NME4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NME4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NME4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050228
Assay Design:   exonic
Chromosome Location:   16:449384-449628question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000397722 ENST00000454619 ENST00000382940 ENST00000219479 ENST00000433358 ENST00000450036

Gene Information

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family which includes NME4 (Milon et al. 1997 [PubMed 9099850]).[supplied by OMIM May 2008]

Gene Symbol:   NME4
Gene Name:   non-metastatic cells 4, protein expressed in
Aliases:   NDPK-D, NM23H4, nm23-H4
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103202
Entrez:   4833
Chromosome Mapping:   16p13.3

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