PrimePCR™ Probe Assay: SEC61B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEC61B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEC61B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050430
Assay Design:   exonic
Chromosome Location:   9:101992663-101992774question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000223641

Gene Information

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha beta and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq Jul 2008]

Gene Symbol:   SEC61B
Gene Name:   Sec61 beta subunit
Aliases:   Not Available
RefSeq:   NC_000009.11 NG_008928.1 NT_008470.19
Ensembl:   ENSG00000106803
Entrez:   10952
Chromosome Mapping:   9q22.32-q31.3

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