PrimePCR™ Probe Assay: VEGFA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VEGFA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VEGFA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050716
Assay Design:   exonic
Chromosome Location:   6:43745231-43745321question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000372067 ENST00000324450 ENST00000417285 ENST00000413642 ENST00000372055 ENST00000482630 ENST00000425836 ENST00000372064 ENST00000372077 ENST00000519767 ENST00000520948 ENST00000523873 ENST00000523950 ENST00000457104 ENST00000518689 ENST00000523125 ENST00000518824 ENST00000230480 ENST00000520265 ENST00000336798

Gene Information

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects including mediating increased vascular permeability inducing angiogenesis vasculogenesis and endothelial cell growth promoting cell migration and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants encoding either freely secreted or cell-associated isoforms have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of and in-frame with the first AUG leading to additional isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   VEGFA
Gene Name:   vascular endothelial growth factor A
Aliases:   MGC70609, MVCD1, VEGF, VPF
RefSeq:   NC_000006.11 NG_008732.1 NT_007592.15
Ensembl:   ENSG00000112715
Entrez:   7422
Chromosome Mapping:   6p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.560000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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