PrimePCR™ Probe Assay: EIF2B4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF2B4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF2B4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050844
Assay Design:   exonic
Chromosome Location:   2:27589786-27590063question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000347454 ENST00000445933 ENST00000451130 ENST00000493344

Gene Information

Eukaryotic initiation factor 2B (EIF2B) which is necessary for protein synthesis is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth or delta subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   EIF2B4
Gene Name:   eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Aliases:   DKFZp586J0119, EIF-2B, EIF2B, EIF2Bdelta
RefSeq:   NC_000002.11 NG_009305.1 NT_022184.15
Ensembl:   ENSG00000115211
Entrez:   8890
Chromosome Mapping:   2p23.3

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