PrimePCR™ Probe Assay: NCF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCF2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050957
Assay Design:   exonic
Chromosome Location:   1:183532621-183533142question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000367536 ENST00000367535 ENST00000420553 ENST00000419402 ENST00000413720 ENST00000418089

Gene Information

This gene encodes neutrophil cytosolic factor 2 the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene as well as in other NADPH oxidase subunits can result in chronic granulomatous disease a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jun 2010]

Gene Symbol:   NCF2
Gene Name:   neutrophil cytosolic factor 2
Aliases:   FLJ93058, NCF-2, NOXA2, P67-PHOX, P67PHOX
RefSeq:   NC_000001.10 NG_007267.1 NT_004487.19
Ensembl:   ENSG00000116701
Entrez:   4688
Chromosome Mapping:   1q25

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