PrimePCR™ Probe Assay: CALD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CALD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CALD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051225
Assay Design:   exonic
Chromosome Location:   7:134617960-134618075question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000417172 ENST00000436461 ENST00000422748 ENST00000361675 ENST00000361901 ENST00000424922 ENST00000495522 ENST00000361388 ENST00000393118 ENST00000543443

Gene Information

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin actin tropomyosin myosin and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CALD1
Gene Name:   caldesmon 1
Aliases:   CDM, H-CAD, HCAD, L-CAD, LCAD, MGC21352, NAG22
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000122786
Entrez:   800
Chromosome Mapping:   7q33

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