PrimePCR™ Probe Assay: GUCY2D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GUCY2D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GUCY2D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051706
Assay Design:   exonic
Chromosome Location:   17:7918212-7918341question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000254854

Gene Information

This gene encodes a retina-specific guanylate cyclase which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain a single membrane spanning domain a kinase homology domain and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq Dec 2008]

Gene Symbol:   GUCY2D
Gene Name:   guanylate cyclase 2D, membrane (retina-specific)
Aliases:   CORD5, CORD6, CYGD, GUC1A4, GUC2D, LCA, LCA1, RCD2, RETGC-1, ROS-GC1, ROSGC, retGC
RefSeq:   NC_000017.10 NG_009092.1 NT_010718.16
Ensembl:   ENSG00000132518
Entrez:   3000
Chromosome Mapping:   17p13.1

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