PrimePCR™ Probe Assay: MDM2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MDM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MDM2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051885
Assay Design:   exonic
Chromosome Location:   12:69233407-69233529question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000462284 ENST00000258148 ENST00000393410 ENST00000299252 ENST00000360430 ENST00000517852 ENST00000545204 ENST00000393413 ENST00000350057 ENST00000348801 ENST00000478070 ENST00000258149 ENST00000356290 ENST00000540827 ENST00000428863 ENST00000393412

Gene Information

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53 as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53 diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle apoptosis and tumorigenesis through interactions with other proteins including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq Jul 2008]

Gene Symbol:   MDM2
Gene Name:   Mdm2 p53 binding protein homolog (mouse)
Aliases:   HDMX, MGC5370, MGC71221, hdm2
RefSeq:   NC_000012.11 NG_016708.1 NT_029419.12
Ensembl:   ENSG00000135679
Entrez:   4193
Chromosome Mapping:   12q14.3-q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.390000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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