PrimePCR™ Probe Assay: TLE3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TLE3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TLE3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052219
Assay Design:   exonic
Chromosome Location:   15:70344850-70345691question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000558939 ENST00000317509 ENST00000560589 ENST00000559929 ENST00000559048 ENST00000560939 ENST00000557997 ENST00000539550 ENST00000558201 ENST00000440567 ENST00000559191 ENST00000557907 ENST00000558379 ENST00000451782 ENST00000442299

Gene Information

Description Not Available

Gene Symbol:   TLE3
Gene Name:   transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Aliases:   ESG, ESG3, FLJ39460, GRG3, HsT18976, KIAA1547
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140332
Entrez:   7090
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.250000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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