PrimePCR™ Probe Assay: EIF4E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF4E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF4E, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052768
Assay Design:   exonic
Chromosome Location:   4:99802096-99802229question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000450253 ENST00000280892 ENST00000511644 ENST00000504432 ENST00000505992

Gene Information

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   EIF4E
Gene Name:   eukaryotic translation initiation factor 4E
Aliases:   CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000151247
Entrez:   1977
Chromosome Mapping:   4q21-q25

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