PrimePCR™ Probe Assay: BUB1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BUB1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BUB1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053015
Assay Design:   exonic
Chromosome Location:   15:40500866-40501852question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000287598 ENST00000412359

Gene Information

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C) delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   BUB1B
Gene Name:   budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Aliases:   BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1
RefSeq:   NC_000015.9 NG_016338.1 NT_010194.17
Ensembl:   ENSG00000156970
Entrez:   701
Chromosome Mapping:   15q15

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