PrimePCR™ Probe Assay: ADAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ADAR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADAR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053167
Assay Design:   exonic
Chromosome Location:   1:154562303-154562398question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000368474 ENST00000368471 ENST00000529168 ENST00000292205

Gene Information

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2010]

Gene Symbol:   ADAR
Gene Name:   adenosine deaminase, RNA-specific
Aliases:   ADAR1, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136
RefSeq:   NC_000001.10 NG_011844.1 NT_004487.19
Ensembl:   ENSG00000160710
Entrez:   103
Chromosome Mapping:   1q21.1-q21.2

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