PrimePCR™ Probe Assay: MCM7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MCM7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MCM7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053651
Assay Design:   exonic
Chromosome Location:   7:99696669-99696760question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000343023 ENST00000303887 ENST00000425308 ENST00000354230

Gene Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2 4 and 6 proteins possesses DNA helicase activity and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase CDK4 is found to associate with this protein and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   MCM7
Gene Name:   minichromosome maintenance complex component 7
Aliases:   CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146
RefSeq:   NC_000007.13 NG_016312.1 NT_007933.15
Ensembl:   ENSG00000166508
Entrez:   4176
Chromosome Mapping:   7q21.3-q22.1

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