PrimePCR™ Probe Assay: KRT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054199
Assay Design:   exonic
Chromosome Location:   12:53038662-53038769question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000309680

Gene Information

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT2
Gene Name:   keratin 2
Aliases:   CK-2e, K2e, KRT2A, KRT2E, KRTE, MGC116967, MGC116968
RefSeq:   NC_000012.11 NG_008296.1 NT_029419.12
Ensembl:   ENSG00000172867
Entrez:   3849
Chromosome Mapping:   12q13.13

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