PrimePCR™ Probe Assay: CSF1R, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CSF1R, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSF1R, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054798
Assay Design:   exonic
Chromosome Location:   5:149460330-149460452question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000286301 ENST00000511344 ENST00000543093

Gene Information

The protein encoded by this gene is the receptor for colony stimulating factor 1 a cytokine which controls the production differentiation and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. [provided by RefSeq Jul 2008]

Gene Symbol:   CSF1R
Gene Name:   colony stimulating factor 1 receptor
Aliases:   C-FMS, CD115, CSFR, FIM2, FMS
RefSeq:   NG_023367.1 NC_000005.9 NG_012303.1 NG_021389.1 NT_029289.11
Ensembl:   ENSG00000182578
Entrez:   1436
Chromosome Mapping:   5q32

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