PrimePCR™ Probe Assay: SULT1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SULT1A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SULT1A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055352
Assay Design:   exonic
Chromosome Location:   16:28617254-28617482question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000533150 ENST00000335715 ENST00000395630 ENST00000360423 ENST00000565290 ENST00000344620 ENST00000395400 ENST00000350842 ENST00000569554 ENST00000314752 ENST00000566189 ENST00000395609 ENST00000395607 ENST00000338971 ENST00000395138 ENST00000563322 ENST00000354723 ENST00000355544 ENST00000395137 ENST00000409039 ENST00000542454 ENST00000554311 ENST00000539359 ENST00000306005 ENST00000521180 ENST00000319914 ENST00000399640 ENST00000374736 ENST00000428484 ENST00000354639 ENST00000433467 ENST

Gene Information

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones neurotransmitters drugs and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SULT1A1
Gene Name:   sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
Aliases:   HAST1/HAST2, MGC131921, MGC5163, P-PST, PST, ST1A1, ST1A3, STP, STP1, TSPST1
RefSeq:   NC_000016.9 NT_010393.16 NG_028128.1
Ensembl:   ENSG00000196502
Entrez:   6817
Chromosome Mapping:   16p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995900
y-intercept 37.660000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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