PrimePCR™ Probe Assay: PABPC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PABPC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PABPC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057840
Assay Design:   exonic
Chromosome Location:   8:101724881-101724976question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000288350 ENST00000358268 ENST00000380671 ENST00000318607 ENST00000519004 ENST00000522387 ENST00000517403 ENST00000519100 ENST00000569397 ENST00000562709 ENST00000281589

Gene Information

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq Aug 2010]

Gene Symbol:   PABPC1
Gene Name:   poly(A) binding protein, cytoplasmic 1
Aliases:   PAB1, PABP, PABP1, PABPC2, PABPL1
RefSeq:   NC_000008.10 NG_027520.1 NT_008046.16
Ensembl:   ENSG00000070756
Entrez:   26986
Chromosome Mapping:   8q22.2-q23

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