PrimePCR™ Probe Assay: REST, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: REST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: REST, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058290
Assay Design:   exonic
Chromosome Location:   4:57777455-57777599question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000309042

Gene Information

This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq Jul 2010]

Gene Symbol:   REST
Gene Name:   RE1-silencing transcription factor
Aliases:   NRSF, XBR
RefSeq:   NC_000004.11 NT_022853.15
Ensembl:   ENSG00000084093
Entrez:   5978
Chromosome Mapping:   4q12

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