PrimePCR™ Probe Assay: GABRA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GABRA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GABRA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026254
Assay Design:   Intron-spanning
Chromosome Location:   5:161281176-161292758question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000023897 ENST00000428797 ENST00000393943 ENST00000437025 ENST00000521339 ENST00000522651 ENST00000519621 ENST00000420560 ENST00000444819

Gene Information

This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric consisting of proteins from several subunit classes: alpha beta gamma delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   GABRA1
Gene Name:   gamma-aminobutyric acid (GABA) A receptor, alpha 1
Aliases:   ECA4, EJM, EJM5
RefSeq:   NC_000005.9 NG_011548.1 NT_023133.13
Ensembl:   ENSG00000022355
Entrez:   2554
Chromosome Mapping:   5q34-q35

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