PrimePCR™ Probe Assay: UBE2J1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: UBE2J1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBE2J1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026282
Assay Design:   Intron-spanning
Chromosome Location:   6:90039544-90042849question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000435041 ENST00000536477

Gene Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s ubiquitin-conjugating enzymes or E2s and ubiquitin-protein ligases or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq Jul 2008]

Gene Symbol:   UBE2J1
Gene Name:   ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
Aliases:   HSPC153, HSPC205, HSU93243, MGC12555, NCUBE-1, NCUBE1, Ubc6p
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000198833
Entrez:   51465
Chromosome Mapping:   6q15

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