PrimePCR™ Probe Assay: FASN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FASN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FASN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026813
Assay Design:   Intron-spanning
Chromosome Location:   17:80051600-80053255question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000306749 ENST00000545909

Gene Information

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA in the presence of NADPH into long-chain saturated fatty acids. In some cancer cell lines this protein has been found to be fused with estrogen receptor-alpha (ER-alpha) in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq Jul 2008]

Gene Symbol:   FASN
Gene Name:   fatty acid synthase
Aliases:   FAS, MGC14367, MGC15706, OA-519, SDR27X1
RefSeq:   NC_000017.10 NT_010663.15
Ensembl:   ENSG00000169710
Entrez:   2194
Chromosome Mapping:   17q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.400000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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