PrimePCR™ Probe Assay: KIT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KIT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KIT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026913
Assay Design:   Intron-spanning
Chromosome Location:   4:55524195-55561716question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000288135 ENST00000412167 ENST00000536403

Gene Information

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors mast cell disease acute myelogenous lukemia and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KIT
Gene Name:   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Aliases:   C-Kit, CD117, PBT, SCFR
RefSeq:   NC_000004.11 NG_007456.1 NT_022853.15
Ensembl:   ENSG00000157404
Entrez:   3815
Chromosome Mapping:   4q11-q12

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