PrimePCR™ Probe Assay: ARNT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARNT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARNT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027033
Assay Design:   Intron-spanning
Chromosome Location:   1:150804824-150807099question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000358595 ENST00000354396 ENST00000515192 ENST00000505755 ENST00000394700 ENST00000368975

Gene Information

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand which includes dioxin and polycyclic aromatic hydrocarbons results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation which results in a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2010]

Gene Symbol:   ARNT
Gene Name:   aryl hydrocarbon receptor nuclear translocator
Aliases:   HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2
RefSeq:   NG_011848.1 NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143437
Entrez:   405
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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