PrimePCR™ Probe Assay: ITPA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ITPA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ITPA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027561
Assay Design:   Intron-spanning
Chromosome Location:   20:3194631-3199216question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000380113 ENST00000399838 ENST00000455664

Gene Information

The protein encoded by this gene hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein which is a member of the HAM1 NTPase protein family is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene. Also at least two other transcript variants have been identified which are probably regulatory rather than protein-coding. [provided by RefSeq Jul 2008]

Gene Symbol:   ITPA
Gene Name:   inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Aliases:   C20orf37, HLC14-06-P, ITPase, dJ794I6.3
RefSeq:   NC_000020.10 NG_012093.1 NT_011387.8
Ensembl:   ENSG00000125877
Entrez:   3704
Chromosome Mapping:   20p

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