PrimePCR™ Probe Assay: PPP2R1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPP2R1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP2R1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027632
Assay Design:   Intron-spanning
Chromosome Location:   19:52724351-52725478question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000423369 ENST00000436460 ENST00000444322 ENST00000322088 ENST00000391791

Gene Information

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme which is composed of a catalytic subunit and a constant regulatory subunit that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq Apr 2010]

Gene Symbol:   PPP2R1A
Gene Name:   protein phosphatase 2, regulatory subunit A, alpha
Aliases:   MGC786, PP2A-Aalpha, PP2AAALPHA, PR65A
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105568
Entrez:   5518
Chromosome Mapping:   19q13.41

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