PrimePCR™ Probe Assay: NNT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NNT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NNT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027689
Assay Design:   Intron-spanning
Chromosome Location:   5:43659368-43675690question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000264663 ENST00000344920 ENST00000512996 ENST00000542759

Gene Information

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NNT
Gene Name:   nicotinamide nucleotide transhydrogenase
Aliases:   MGC126502, MGC126503
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000112992
Entrez:   23530
Chromosome Mapping:   5p12

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