PrimePCR™ Probe Assay: SEC31A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEC31A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEC31A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028237
Assay Design:   Intron-spanning
Chromosome Location:   4:83776117-83778195question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000348405 ENST00000513858 ENST00000395310 ENST00000443462 ENST00000509142 ENST00000505472 ENST00000500777 ENST00000508502 ENST00000355196 ENST00000264405 ENST00000505984 ENST00000508479 ENST00000507828 ENST00000512664 ENST00000510167 ENST00000432794 ENST00000448323 ENST00000326950 ENST00000311785

Gene Information

The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13 one of the other components of COPII in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   SEC31A
Gene Name:   SEC31 homolog A (S. cerevisiae)
Aliases:   ABP125, ABP130, DKFZp686N07171, HSPC334, KIAA0905, MGC90305, SEC31L1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138674
Entrez:   22872
Chromosome Mapping:   4q21.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.640000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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