PrimePCR™ Probe Assay: ENO1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: ENO1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ENO1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028266
Assay Design:   Intron-spanning
Chromosome Location:   1:8923329-8924028question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000234590

Gene Information

This gene encodes alpha-enolase one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha 2 gamma or 2 beta subunits and functions as a glycolytic enzyme. Alpha-enolase in addition functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq Jan 2011]

Gene Symbol:   ENO1
Gene Name:   enolase 1, (alpha)
Aliases:   ENO1L1, MPB1, NNE, PPH
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000074800
Entrez:   2023
Chromosome Mapping:   1p36.2

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