PrimePCR™ Probe Assay: PLAT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLAT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLAT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028495
Assay Design:   Intron-spanning
Chromosome Location:   8:42033605-42036498question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000429089 ENST00000220809 ENST00000352041 ENST00000519510 ENST00000429710 ENST00000524009 ENST00000270189

Gene Information

This gene encodes tissue-type plasminogen activator a secreted serine protease which converts the proenzyme plasminogen to plasmin a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   PLAT
Gene Name:   plasminogen activator, tissue
Aliases:   DKFZp686I03148, T-PA, TPA
RefSeq:   NC_000008.10 NG_023264.1 NT_167187.1
Ensembl:   ENSG00000104368
Entrez:   5327
Chromosome Mapping:   8p12

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