PrimePCR™ Probe Assay: TNNI3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNNI3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNNI3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028574
Assay Design:   Intron-spanning
Chromosome Location:   19:55665477-55666186question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000344887

Gene Information

Troponin I (TnI) along with troponin T (TnT) and troponin C (TnC) is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch TnI-skeletal-slow-twitch and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq Jul 2008]

Gene Symbol:   TNNI3
Gene Name:   troponin I type 3 (cardiac)
Aliases:   CMD1FF, CMD2A, CMH7, MGC116817, RCM1, TNNC1, cTnI
RefSeq:   NC_000019.9 NG_007866.1 NG_011829.1 NT_011109.16
Ensembl:   ENSG00000129991
Entrez:   7137
Chromosome Mapping:   19q13.4

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