PrimePCR™ Probe Assay: PTPRF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPRF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPRF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028797
Assay Design:   Intron-spanning
Chromosome Location:   1:44057548-44058209question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000359947 ENST00000438120 ENST00000412568 ENST00000429895 ENST00000414879 ENST00000372414 ENST00000372413 ENST00000422171

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP possesses an extracellular region a single transmembrane region and two tandem intracytoplasmic catalytic domains and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese insulin-resistant individuals and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene which encode distinct proteins have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPRF
Gene Name:   protein tyrosine phosphatase, receptor type, F
Aliases:   FLJ43335, FLJ45062, FLJ45567, LAR
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000142949
Entrez:   5792
Chromosome Mapping:   1p34

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