PrimePCR™ Probe Assay: TXNRD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TXNRD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TXNRD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028905
Assay Design:   Intron-spanning
Chromosome Location:   12:104719133-104720164question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000525566 ENST00000503506 ENST00000526691 ENST00000526390 ENST00000529546 ENST00000529751 ENST00000524698 ENST00000526950 ENST00000429002 ENST00000388854 ENST00000542918 ENST00000378070 ENST00000397736 ENST00000427956 ENST00000354940 ENST00000540716

Gene Information

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   TXNRD1
Gene Name:   thioredoxin reductase 1
Aliases:   GRIM-12, MGC9145, TR, TR1, TRXR1, TXNR
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000198431
Entrez:   7296
Chromosome Mapping:   12q23-q24.1

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