PrimePCR™ Probe Assay: ERBB4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERBB4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERBB4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031507
Assay Design:   Intron-spanning
Chromosome Location:   2:212652836-212812280question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000342788 ENST00000436443 ENST00000260943 ENST00000435846 ENST00000402597

Gene Information

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains a transmembrane domain a tyrosine kinase domain a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   ERBB4
Gene Name:   v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Aliases:   HER4, MGC138404, p180erbB4
RefSeq:   NC_000002.11 NT_005403.17 NG_011805.1
Ensembl:   ENSG00000178568
Entrez:   2066
Chromosome Mapping:   2q33.3-q34

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