PrimePCR™ Probe Assay: KIFAP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KIFAP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KIFAP3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031855
Assay Design:   Intron-spanning
Chromosome Location:   1:169923184-169930298question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000361580 ENST00000367765 ENST00000367767 ENST00000538366 ENST00000540905

Gene Information

The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein which is highly concentrated around the endoplasmic reticulum is phosphorylated by v-src and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B a kinesin superfamily protein in the nucleus and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   KIFAP3
Gene Name:   kinesin-associated protein 3
Aliases:   FLJ22818, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1
RefSeq:   NC_000001.10 NG_012883.2 NT_004487.19
Ensembl:   ENSG00000075945
Entrez:   22920
Chromosome Mapping:   1q24.2

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