PrimePCR™ Probe Assay: CSNK1E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CSNK1E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSNK1E, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033608
Assay Design:   Intron-spanning
Chromosome Location:   22:38696813-38698896question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000359867 ENST00000402865 ENST00000400206 ENST00000403904 ENST00000413574 ENST00000405675 ENST00000451964 ENST00000430335 ENST00000396832

Gene Information

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family whose members have been implicated in the control of cytoplasmic and nuclear processes including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins including itself. This protein has been shown to phosphorylate period a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CSNK1E
Gene Name:   casein kinase 1, epsilon
Aliases:   HCKIE, MGC10398
RefSeq:   NC_000022.10 NG_016707.1 NT_011520.12
Ensembl:   ENSG00000213923
Entrez:   1454
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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