PrimePCR™ Probe Assay: RASGRP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RASGRP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RASGRP3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033882
Assay Design:   Intron-spanning
Chromosome Location:   2:33740134-33741645question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000402538 ENST00000437184 ENST00000403687 ENST00000442390 ENST00000425210 ENST00000444784 ENST00000423159 ENST00000407811

Gene Information

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs) such as RASGRP3 serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al. 2000 [PubMed 10934204]).[supplied by OMIM Mar 2008]

Gene Symbol:   RASGRP3
Gene Name:   RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Aliases:   GRP3, KIAA0846
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000152689
Entrez:   25780
Chromosome Mapping:   2p25.1-p24.1

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