PrimePCR™ Probe Assay: CASP7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CASP7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CASP7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039459
Assay Design:   Intron-spanning
Chromosome Location:   10:115481504-115485208question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000429617 ENST00000369331 ENST00000345633 ENST00000369318 ENST00000369315 ENST00000369321 ENST00000452490

Gene Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits large and small that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants encoding three distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CASP7
Gene Name:   caspase 7, apoptosis-related cysteine peptidase
Aliases:   CMH-1, ICE-LAP3, MCH3
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000165806
Entrez:   840
Chromosome Mapping:   10q25

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