PrimePCR™ Probe Assay: HSPA1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPA1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPA1L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040037
Assay Design:   Intron-spanning
Chromosome Location:   6:31779685-31782727question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000359674 ENST00000395473 ENST00000302228 ENST00000310394 ENST00000383390 ENST00000538767 ENST00000422670 ENST00000375654 ENST00000426095 ENST00000338432 ENST00000377848 ENST00000377854 ENST00000543201 ENST00000350796 ENST00000417601 ENST00000439554 ENST00000276185 ENST00000456772 ENST00000414247 ENST00000233623 ENST00000410003

Gene Information

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq Jul 2008]

Gene Symbol:   HSPA1L
Gene Name:   heat shock 70kDa protein 1-like
Aliases:   HSP70-1L, HSP70-HOM, HSP70T, hum70t
RefSeq:   NC_000006.11 NG_011855.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167248.1
Ensembl:   ENSG00000204390
Entrez:   3305
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996600
y-intercept 36.330000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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