PrimePCR™ Probe Assay: ERCC2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERCC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERCC2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040545
Assay Design:   Intron-spanning
Chromosome Location:   19:45868362-45871982question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000391945 ENST00000391941 ENST00000485403 ENST00000586131 ENST00000586856 ENST00000391940

Gene Information

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders the cancer-prone syndrome xeroderma pigmentosum complementation group D trichothiodystrophy and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   ERCC2
Gene Name:   excision repair cross-complementing rodent repair deficiency, complementation group 2
Aliases:   COFS2, EM9, MGC102762, MGC126218, MGC126219, TTD, XPD
RefSeq:   NC_000019.9 NG_007067.2 NT_011109.16
Ensembl:   ENSG00000104884
Entrez:   2068
Chromosome Mapping:   19q13.3

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