PrimePCR™ Probe Assay: AKR1C1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKR1C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKR1C1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041688
Assay Design:   Intron-spanning
Chromosome Location:   10:5009185-5011029question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000380872 ENST00000442997 ENST00000380859 ENST00000434459 ENST00000380753 ENST00000407674 ENST00000380554 ENST00000439082 ENST00000380448 ENST00000263126 ENST00000578362 ENST00000582412 ENST00000578631 ENST00000584844 ENST00000580545 ENST00000580345 ENST00000585191 ENST00000583876 ENST00000579965 ENST00000583238

Gene Information

This gene encodes a member of the aldo/keto reductase superfamily which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq Jul 2008]

Gene Symbol:   AKR1C1
Gene Name:   aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase)
Aliases:   2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, HBAB, MBAB, MGC8954
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000187134
Entrez:   1645
Chromosome Mapping:   10p15-p14

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