PrimePCR™ Probe Assay: Hsd3b3, Mouse

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: Hsd3b3, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: Hsd3b3, Mouse
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qMmuCEP0043354
Assay Design:		exonic
Chromosome Location:		3:98753311-98753434question
Amplicon Length:		94
Splice Variants Targeted:		ENSMUST00000143577 ENSMUST00000103122 ENSMUST00000163147 ENSMUST00000052404 ENSMUST00000027632 ENSMUST00000073490 ENSMUST00000032992 ENSMUST00000169701 ENSMUST00000091609 ENSMUST00000140142 ENSMUST00000049575 ENSMUST00000019470 ENSMUST00000114634 ENSMUST00000023370 ENSMUST00000098971 ENSMUST00000054622 ENSMUST00000108814 ENSMUST00000048608 ENSMUST00000108815 ENSMUST00000148252 ENSMUST00000107019 ENSMUST00000107018 ENSMUST00000148578 ENSMUST00000135706 ENSMUST00000125483 ENSMUST00000146196 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Hsd3b3
Gene Name:		Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3
Aliases:		9030618K22Rik, AI790201
RefSeq:		NC_000069.6 NT_039240.8
Ensembl:		ENSMUSG00000062410
Entrez:		15494
Chromosome Mapping:		3 F2.2\|3 49.1 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.480000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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