PrimePCR™ Probe Assay: Cldn18, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cldn18, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cldn18, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056055
Assay Design:   exonic
Chromosome Location:   9:99698103-99698823question
Amplicon Length:   80
Splice Variants Targeted:   ENSMUST00000035048 ENSMUST00000112882 ENSMUST00000136429 ENSMUST00000131922

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. This gene is a downstream target gene regulated by the T/EBP/NKX2.1 homeodomain transcription factor. Four alternatively spliced transcript variants resulted from alternative promoters and alternative splicing have been identified which encode two lung-specific isoforms and two stomach-specific isoforms respectively. This gene is also expressed in colons inner ear and skin and its expression is increased in both experimental colitis and ulcerative colitis. [provided by RefSeq Aug 2010]

Gene Symbol:   Cldn18
Gene Name:   Claudin 18
Aliases:   Not Available
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000032473
Entrez:   56492
Chromosome Mapping:   9 E3-F1|9

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