PrimePCR™ Probe Assay: Gli3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gli3, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gli3, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026742
Assay Design:   intron_spanning
Chromosome Location:   17:50451089-50475922question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000019396

Gene Information

transcription factor; member of the C2H2-type zinc finger protein subclass of the Gli family of transcription factors; mediates sonic hedgehog (Shh) signalling; may play a role in embryogenesis [RGD, Feb 2006]

Gene Symbol:   Gli3
Gene Name:   GLI-Kruppel family member GLI3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000014395
Entrez:   140588
Chromosome Mapping:   17q12.1

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