PrimePCR™ Probe Assay: Prkaca, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Prkaca, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Prkaca, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0027821
Assay Design:   intron_spanning
Chromosome Location:   19:36086607-36090442question
Amplicon Length:   180
Splice Variants Targeted:   ENSRNOT00000041717 ENSRNOT00000007281 ENSRNOT00000034574

Gene Information

active catalytic subunit of protein kinase A; phosphorylates and activates transcription factors in response to elevated levels of cAMP; mediates phosphorylation and regulation of enzyme substrates involved in intermediary metabolism [RGD, Feb 2006]

Gene Symbol:   Prkaca
Gene Name:   CAMP-dependent protein kinase catalytic subunit alpha
Aliases:   Not Available
RefSeq:   NM_001100922
Ensembl:   ENSRNOG00000005257
Entrez:   25636
Chromosome Mapping:   19q11

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