PrimePCR™ Probe Assay: PSEN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSEN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSEN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058435
Assay Design:   exonic
Chromosome Location:   14:73603283-73614687question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000557356 ENST00000556864 ENST00000556533 ENST00000556951 ENST00000557293 ENST00000553719 ENST00000553599 ENST00000556011 ENST00000394157 ENST00000357710 ENST00000324501 ENST00000560005 ENST00000555254 ENST00000554131 ENST00000557037 ENST00000394164 ENST00000556066 ENST00000261970 ENST00000344094

Gene Information

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase an enzyme that cleaves APP. Also it is thought that the presenilins are involved in the cleavage of the Notch receptor such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene the full-length nature of only some have been determined. [provided by RefSeq Aug 2008]

Gene Symbol:   PSEN1
Gene Name:   presenilin 1
Aliases:   AD3, FAD, PS-1, PS1, S182
RefSeq:   NC_000014.8 NG_007386.2 NT_026437.12
Ensembl:   ENSG00000080815
Entrez:   5663
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 35.500000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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