PrimePCR™ Probe Assay: HLA-C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040063
Assay Design:   exonic
Chromosome Location:   6:31236823-31237124question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000592696 ENST00000379803 ENST00000248054 ENST00000367582 ENST00000440869 ENST00000305766 ENST00000427704 ENST00000367584 ENST00000316615 ENST00000316804 ENST00000392562 ENST00000392564 ENST00000392563 ENST00000580599 ENST00000383487 ENST00000383483 ENST00000458668 ENST00000400341 ENST00000341901 ENST00000376228 ENST00000383329 ENST00000539307 ENST00000356786 ENST00000453809 ENST00000414249 ENST00000419590 ENST00000438171 ENST00000552865 ENST00000541377 ENST00000419960 ENST00000448504 ENST

Gene Information

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide exons 2 and 3 encode the alpha1 and alpha2 domain which both bind the peptide exon 4 encodes the alpha3 domain exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-C
Gene Name:   major histocompatibility complex, class I, C
Aliases:   D6S204, FLJ27082, HLA-Cw, HLA-JY3, HLC-C, PSORS1
RefSeq:   NT_167245.1 NT_167248.1 NT_167249.1 NC_000006.11 NG_002397.2 NT_007592.15 NT_113891.2 NT_167246.1 NT_167247.1
Ensembl:   ENSG00000204525
Entrez:   3107
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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