PrimePCR™ Probe Assay: MKNK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MKNK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MKNK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029303
Assay Design:   Intron-spanning
Chromosome Location:   19:2043557-2046254question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000309340 ENST00000250896 ENST00000545627

Gene Information

This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E) thus playing important roles in the initiation of mRNA translation oncogenic transformation and malignant cell proliferation. In addition to eIF4E this protein also interacts with von Hippel-Lindau tumor suppressor (VHL) ring-box 1 (Rbx1) and Cullin2 (Cul2) which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation and in subcellular localization. [provided by RefSeq Aug 2011]

Gene Symbol:   MKNK2
Gene Name:   MAP kinase interacting serine/threonine kinase 2
Aliases:   GPRK7, MNK2
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000099875
Entrez:   2872
Chromosome Mapping:   19p13.3

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